Detailed information about the course

[ Back ]
Title

Crispr Cas9

Dates

12-14 mars 2019

Responsable de l'activité

Pilar Junier

Organizer(s)
Speakers

Prof. Anil Wipat, Newcastle University Dre Valeria Verrone, Newcastle University 

Description

 

An introduction to CRISPR-Cas9: A focus on bacteria

 

Although a relatively recent discovery, CRISPR technology is beginning to revolutionise biotechnology. This workshop is designed for beginners to the field to introduce scientists to the world of CRISPR by providing a summary of the fundamentals. The workshop will comprise lectures, workshops and laboratory-based exercises. The lectures cover the history and theory of CRISPR, how the technology works, the wide variety of CRISPR applications, and approaches to carrying out your own CRISPR experiment, with a special focus on engineering bacteria. A computational workshop will demonstrate how guide RNA for CRISPR experiments can be designed. The practical component of the course in the lab will demonstrate how dCas9 based CRISPR can be used to knock down bacterial gene expression.

 

Program

 

 

Trainers: Anil Wipat & Valeria Verrone

 

Program

 

Tuesday 12th March 2019 – Room E301

09:00-10:30
Lectures on background of the technology, underlying biology, practical issues and applications focusing
on applications of CRISPR-Cas9 in microbiology. 

10:30-11:00 Coffee break

11:00-12:00
Interactive session about the practical: creating a KO cadA using CRISPR on Escherichia coli

12:00-14:00
Lunch break (lunch for the group at the cafeteria of UniMail)

14:00-15:00
Computational practical in Guide RNA (please bring your computer)

15:00-15:30
Coffee break

15:30-17:00
Cont. Computational practical in Guide RNA

18:00
Dinner with the speakers (please let us know in advance if you would like to join for this activity).

 

Wednesday 13th March 2019 – Room TP4 D008 

09:00-10:30
Preparing competent cells 

10:30-11:00
Coffee break

11:00-12:00 Cont. Preparing competent cells.

12:00-14:00
Lunch break (lunch for the group at the cafeteria of UniMail). 

14:00-15:00
Transforming the strains. 

15:00-15:30 C
offee break

15:30-17:00
Cont. Transforming the strains and discussion of the theoretical steps that follow.

 

Thursday 14th March 2019 – Room TP4 D008 

09:00-10:30
Assays to verified the phenotype.

10:30-11:00
Coffee break

11:00-12:00
Cont. Assays to verified the phenotype and discussion of the overall activity.

 

Location

Neuchâtel

Information

 

The workshop will take place at the university of Neuchâtel in the following rooms:

 

12.3: E301 

 

13.3: TP4 (D008)

 

14.3: TP4 (D008) 

 

Places

15

Deadline for registration 07.03.2019
Contact

microbiologie@cuso.ch

short-url short URL

short-url URL onepage