[ Back ]


Establishment of the CRISPR-CAS system for rapid diagnostics and the development of antiviral strategies

Author Damian JANDRASITS
Director of thesis Michael Hothorn
Co-director of thesis Mauro Tonolla
Summary of thesis

We aim to use the CRISPR-Cas system for specific and sensitive detection of west nile virus in infected samples. For this we will use the Cas13a protein, which has a collateral cleavage activity, which will be used for the readout via lateral flow assay. This assay can be read out by pure eye, and by using a camera of a smartphone we could analyse and evaluate the results.

Another goal of the thesis is to perform a genome-scale CRISPR/Cas9 Knockout screen, in order to identify essential genes for an infection with the crimean-congo hemorrhagic fever virus, which is a threat to human health. Afterwards, specific molecules targeting these genes identified in the CRISPR-Cas9 screen could be evaluated on their efficiency in preventing or treating such an infection, thereby offering the chance to discover new drugs to combat this virus.

Status beginning
Administrative delay for the defence 2025