Electron Microscopy (EM) and Cryo-EM - Practical Course

3-4 & 10-11 November, 2025

Christel Genoud, Dr., Electron Microscopy Facility, UNIL

Christel Genoud, Dr., Electron Microscopy Facility, UNIL

Damien DeBellis, Electron Microscopy Facility, UNIL

Antonio Mucciolo, Electron Microscopy Facility, UNIL

Alexandra Schmitt, EM expert, EMF, UNIL

Amanda Lewis, Dr., EPFL, expert CLEM

Alexandre Myasnikov, DCI, UNIL EPFL

Elsa Natividad Astoria Simon, Navarro lab, UNIL

Bertrand Beckert, DCI,UNIL EPFL

Emiko Uchikawa, DCI, UNIL EPFL

Sergei Nazarov, DCI UNIL EPFL

Inay Mohammed, DCI, UNIL EPFL

Mornings: lectures

Afternoon: hands-on demonstrations

2 days focusing on classic EM, 2 days on Cryo EM

Goals:

- To discover which EM techniques that are available at the EMF could forward your research

- To identify how molecules can be localized in cells or tissues.

- To learn the basics of EM image acquisition, analysis and reconstruction

- To understand basic SEM and TEM operating principles

- To gain exposure to various methods of preparing biological samples for EM together with the advantages and

disadvantages related to each of these methods

Topics covered:

- TEM, transmission electron microscopy

- cryoTEM

- TEM sample preparation: chemical and high-pressure freezing procedures

- SEM, scanning electron microscopy

- SEM sample preparation

- Volume EM: TEM- tomography, Focused Ion Beam-SEM, Array Tomography

- Ultramicrotomy, resin sections

- Cryo-ultramicrotomy, the Tokuyasu technique

- Image processing, volume reconstruction, and modelling

> On the first day of activity, each student has 3 minutes (1-2 slides) to explain his needs in electron microscopy in the frame of the PhD work.

See here the Full Program (PDF)

Location:

3 & 4 November 2025Mornings: Room 340.1, Amphipôle Building

Afternoons: EMF, 1st floor, Biophore Building

10 & 11 November 2025

Mornings: Room 334, Amphipôle Building

Afternoons: EMF, 1st floor, Biophore Building

University of Lausanne, 340.1 & 334 Amphipôle

This course is organized in collaboration with the CUSO doctoral programs in Microbial Sciences and StarOmics

Reimbursements for CUSO MPS Students: Train ticket, 2°class, half-fare from your institution to the place of the activity.

Reimbursement of your travel tickets can be asked online through your MyCUSO. See HERE for the procedure.

For any question concerning reimbursement please contact the CUSO MPS coordinator Debora Zoia

Other CUSO Students: Please contact the coordinator of your Program

Deadline for registration: 20.10.2025

12 places available for CUSO students (4 for each participating program)

Minimum number of participants: 10

For cancellations after the deadline of registration or no show: 150 CHF administrative fees

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