Plant imaging: Advanced Light Microscopy

30-31 January 2025

Sylvain Loubéry, Dr., Plant Imaging Unit, UNIGE

Sylvain Loubéry, Dr., Plant Imaging Unit, UNIGE

The course will focus on light microscopy, and the objective of the course is two-fold:
1) to acquire or strengthen basic knowledge of the most common methods of plant optical imaging (including sample preparation and staining);
2) to acquire or strengthen basic skills in image acquisition and in image analysis.

The course will include two lectures on basic and advanced fluorescence microscopy and sample preparation. In parallel, various practical sessions will be held:
• hands-on sessions on fluorescence microscopes;
• a hands-on session on image analysis;
• an introductory session on plant histology (sectioning with a microtome and a vibratome, staining and mounting).

For the microscopy and histology practical sessions, participants will be grouped by 3 and the groups will rotate between different setups. These sessions will be done with samples brought by the participants. During the image analysis hands-on, participants will work on the software Fiji; this will be done on the participants' personal laptops. During the microscopy sessions, all participants will use three different setups, including widefield as well as CLSM confocal microscopes.

The participants are expected to have prior experience with microscopy (and preferably with confocal microscopy).
Participants will be asked to bring their own laptops for the image analysis hand-on session.

The practicals will take place at the Bioimaging Center of UniGE.

> Flyer (PDF)

> Program (PDF)

University of Geneva, building Sciences III Room 0059

The participants will bring their own samples and their own laptops for this course.
The practicals will take place at the Bioimaging Center of UniGE.

The course is organized in collaboration with the Doctoral School in Life Sciences of Neuchâtel (DSLS)

Reimbursements for CUSO MPS Students: Train ticket, 2°class, half-fare from your institution to the place of the activity.

Reimbursement of your travel tickets can be asked online through your MyCUSO. See HERE for the procedure.

For any question concerning reimbursement please contact the CUSO MPS coordinator Debora Zoia
Reimbursement for DSLS Students: please contact Emilie Genty (DSLS coordinator)

- Maximum number of participants: 9 people (minimum 6).
- 5 places available for CUSO MPS students, 4 places available for DSLS students.
- A waiting list will be prepared to ensure that there are no empty seats.

Deadline for registration: 15 January 2025

*For cancellations after the deadline of registration or no-show: 50 CHF administrative fee

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