Electron Microscopy - Practical Course

9-13 October 2023

Christel Genoud, Dr., Electron Microscopy Facility, University of Lausanne

Christel Genoud, head of EM platform UNIL

Jean Daraspe, Expert scientist EMF UNIL, expert FIBSEM tomography

Jacob Hoogenboom; invited professor from Delft University, expert EM method development

Céline Loussert, expert cryo-immuno, CSEM, Neuchâtel

Iva Garneva, post-doc, gr W. Kukulski, UNIBE

Graham Knott, head of bio-EM facility, SV, EPFL

Amanda Lewis, post-doc EPFL, expert CLEM in human neurodegenerative diseases

Benjamin Boury jamot, imaging specialist, CEC, CHUV,

Alexandre Myasnikov, head of Dubochet Center for Imaging, expert in cryo-EM

Cristina Martin Olmos, head cryo-SEM, EPFL,

Ulrich Lorenz, Professor, Laboratory of molecular nanodymanics, LND; EPFL

Practicals:

Damien DeBellis, EMF, UNIL

Wei Jiao, EMF, UNIL

Introduction to TEM : Jean Daraspe, EMF, UNIL

Introduction to SEM : Antonio Mucciolo, EMF, UNIL; Cristina Martin Olmos, EMF, UNIL

The course with take place at the Electron Microscopy Facility of The University of Lausanne.

Mornings: lectures

Afternoon: hands-on demonstrations + work on your own sample

Goals:

- To identify the best EM method for each student samples/questions

- To discover which EM techniques that are available at the EMF could forward your research

- To identify how molecules can be localized in cells or tissues.

- To learn the basics of EM image acquisition, analysis and reconstruction

- To understand basic SEM and TEM operating principles

- To gain exposure to various methods of preparing biological samples for EM together with the advantages and disadvantages related to each of these methods

Topics covered:

- TEM, transmission electron microscopy

- TEM sample preparation: chemical and high-pressure f freezing procedures

- SEM, scanning electron microscopy

- SEM sample preparation

- Volume EM: TEM- tomography, Focused Ion Beam-SEM, Array Tomography

- Ultramicrotomy, resin sections - Cryo-ultramicrotomy, the Tokuyasu technique

- Image processing, volume reconstruction, and modelling

> On Monday morning, each student has 3 minutes (1-2 slides) to explain his needs in electron microscopy in the frame of the PhD work.

Program (PDF)

Electron Microscopy Facility (EMF), University of Lausanne

This course is organized in collaboration with the CUSO doctoral programs in Microbial Sciences and StarOmics.

Reimbursements for CUSO MPS Students: 

- Train ticket, 2°class, half-fare from your institution to the place of the activity.

Reimbursement of your travel tickets can be asked online through your MyCUSO. See HERE for the procedure.

For any question concerning reimbursement please contact the CUSO MPS coordinator Debora Zoia

Other CUSO Students:

Please contact the coordinator of your Program.

Deadline for registration: 25.09.2023

12 places available for CUSO students (4 for each participating program)

For cancellations after the 25.09.2023 or no show: 150 CHF administrative fees

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