Electron Microscopy - Practical Course

9-13 October 2023

Dre Christel Genoud, UNIL
M. Jean Daraspe, UNIL
M. Antonio Mucciolo, UNIL
M. Damien De Bellis, UNIL
Mme Irina Kolotuev, Senior scientist UNIL, expert
Mme Olivia Muriel Lopez, EMF, UNIL

+ external experts, to be confirmed

Mornings: lectures

Afternoons: hands-on demonstrations + work on your own sample Goals:

- To identify the best EM method for each student samples/questions;
- To discover which EM techniques that are available at the EMF could forward your research;
- To identify how molecules can be localized in cells or tissues;
- To learn the basics of EM image acquisition, analysis and reconstruction;
- To understand basic SEM and TEM operating principles;
- To gain exposure to various methods of preparing biological samples for EM together with the advantages and disadvantages related to each of these methods

Topics covered:
- TEM, transmission electron microscopy;
- TEM sample preparation: chemical and high-pressure freezing procedures;
- SEM, scanning electron microscopy;
- SEM sample preparation;
- Volume EM: TEM- tomography, Focused Ion Beam-SEM, Array Tomography;
- Ultramicrotomy, resin sections;
- Cryo-ultramicrotomy, the Tokuyasu technique;
- Image processing, volume reconstruction, and modelling

> On Monday morning, each student has 3 minutes (1-2 slides) to explain his needs in electron microscopy in the frame of the PhD work.

MORE INFORMATION WILL FOLLOW

Electron Microscopy Facility (EMF), University of Lausanne

This course is organized in collaboration with the CUSO doctoral programs in Microbial Sciences and Molecular Plants Sciences.

Reimbursements:

Reimbursements for CUSO StarOmics students: - Train ticket, 2°class, half-fare from your institution to the place of the activity.

NEW from 2021: Reimbursement of your travel tickets can be asked online through your MyCUSO.

See HERE for the procedure.

For any question concerning reimbursement please contact the CUSO StarOmics coordinator Corinne Dentan

Other CUSO Students: Please contact the coordinator of your Program.

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Deadline for registration: TBA

12 places available for CUSO students (4 for each participating program)

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