Electron Microscopy (EM) and Cryo-EM - Practical Course

Spring-Autumn 2025

Dre Christel Genoud, UNIL Jean Daraspe, UNIL Antonio Mucciolo, UNIL Damien De Bellis, UNIL 2-4 intervenant·es externes

Mornings: lectures

Afternoon: hands-on demonstrations + work on your own sample 2 days focusing on classic EM, 2 days on Cryo EM Goals: to identify the best EM method for each student samples/questions; to discover which EM techniques that are available at the EMF could forward your research; to identify how molecules can be localized in cells or tissues; to learn the basics of EM image acquisition, analysis and reconstruction; to understand basic SEM and TEM operating principles; to gain exposure to various methods of preparing biological samples for EM together with the advantages and disadvantages related to each of these methods.

Topics covered: TEM, transmission electron microscopy; cryoTEM; TEM sample preparation: chemical and high-pressure freezing procedures; SEM, scanning electron microscopy; SEM sample preparation; Volume EM: TEM- tomography, Focused Ion Beam-SEM, Array Tomography; Ultramicrotomy, resin sections; Cryo-ultramicrotomy, the Tokuyasu technique; Image processing, volume reconstruction, and modelling.

UNIL

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