[ANNULÉ] Electron Microscopy (EM) and Cryo-EM - Practical Course

28 & 29 October, 4 & 5 November 2024 (4 days)

Christel Genoud, Dr., Electron Microscopy Facility (EMF), University of Lausanne

Christel Genoud, head of EM Platform, University of Lausanne (UNIL)
Jean Daraspe, EMF, University of Lausanne
Benjamin Boury-Jamot, University of Lausanne
Amanda Lewis, Dr., EPFL
Alexander Myasnikov, head of Dubochet Center for Imaging (DCI), Lausanne
Cristina Martin Olmos, head Cryo-SEM, EPFL
Bertrand Beckert, DCI, UNIL & EPFL
Emiko Uchikawa, DCI, UNIL & EPFL
Sergei Nazarov, DCI, UNIL & EPFL
Inay Mohammed, DCI, UNIL & EPFL

The course with take place at the University of Lausanne. The practicals will be held at the Electron Microscopy Facility of The University of Lausanne.

Mornings: lectures

Afternoon: hands-on demonstrations + work on participant's samples

2 days will be dedicated on classic EM and 2 days on Cryo EM

Goals:
* identify the best EM method for each student samples/questions;
* discover which EM techniques that are available at the EMF could forward your research;
* identify how molecules can be localized in cells or tissues;
* learn the basics of EM image acquisition, analysis and reconstruction;
* understand basic SEM and TEM operating principles & gain exposure to various methods of preparing biological samples for EM together with the advantages and disadvantages related to each of these methods.

Topics covered:
TEM, transmission electron microscopy;
cryoTEM;
TEM sample preparation: chemical and high-pressure freezing procedures;
SEM, scanning electron microscopy;
SEM sample preparation;
Volume EM: TEM- tomography, Focused Ion Beam-SEM, Array Tomography;
Ultramicrotomy, resin sections;
Cryo-ultramicrotomy, the Tokuyasu technique & Image processing, volume reconstruction, and modelling

> On the first day of activity, each student has 3 minutes (1-2 slides) to explain his needs in electron microscopy in the frame of the PhD work.

Schedule: 9h15 - 16h45

PROGRAM & Information (PDF)

University of Lausanne, rooms 338/340/340a

Reimbursements for CUSO MPS Students:
Train ticket, 2°class, half-fare from your institution to the place of the activity.

Reimbursement of your travel tickets can be asked online through your MyCUSO. 

Deadline for registration: 13.10.2024

12 places available for CUSO students (4 for each participating program)

For cancellations after the deadline of registration or no show: 150 CHF administrative fees

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