Detailed information about the course

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Title

Electron Microscopy - Practical Course

Dates

2-6 October 2023

Organizer(s)
Speakers

Christel Genoud, Dr., Head of the Electron Microscopy Facility (EMF), UNIL
Jean Daraspe, Scientific expert, EMF, UNIL
Irina Kolotuev, Senior scientist UNIL, expert
Antonio Mucciolo, EMF, UNIL
Damien De Bellis, EMF, UNIL
Olivia Muriel Lopez, EMF, UNIL

+ other external experts (TBA)

Description

Mornings: lectures Afternoon: hands-on demonstrations + work on your own sample Goals: - To identify the best EM method for each student samples/questions - To discover which EM techniques that are available at the EMF could forward your research - To identify how molecules can be localized in cells or tissues. - To learn the basics of EM image acquisition, analysis and reconstruction - To understand basic SEM and TEM operating principles - To gain exposure to various methods of preparing biological samples for EM together with the advantages and disadvantages related to each of these methods Topics covered: - TEM, transmission electron microscopy - TEM sample preparation: chemical and high-pressure freezing procedures - SEM, scanning electron microscopy - SEM sample preparation - Volume EM: TEM- tomography, Focused Ion Beam-SEM, Array Tomography - Ultramicrotomy, resin sections - Cryo-ultramicrotomy, the Tokuyasu technique - Image processing, volume reconstruction, and modelling

Program

> On Monday morning, each student has 3 minutes (1-2 slides) to explain his needs in electron microscopy in the frame of the PhD work.

 

MORE INFORMATION WILL FOLLOW

Location

University of Lausanne

Information
Registration

Registration here

Deadline for registration: TBA
4 places available for CUSO Microbiology students

Places

12

Deadline for registration
Joint activity joint
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