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Title

Structural and functional characterization of eukaryotic SPX domains acting as molecular phosphate sensors

Author Rebekka WILD
Director of thesis Prof. Michael Hothorn
Co-director of thesis
Summary of thesis SPX domains, which are present in all eukaryotes, are found in proteins functionally associated with phosphate/polyphosphate metabolism, transport, storage and signaling and are perhaps acting as a molecular phosphate sensor. However, their molecular function remains to be elucidated. I have already sucessfully crystallized a yeast and a human SPX domain and the structures indicate a possible phosphate binding pocket. The binding of inorganic phosphate by this small protein module can be analysed via a nuclear magnetic resonance based titration approach. The functionality of structure-based mutants will be investigated using the model plant Arabidopsis thaliana. Elucidating the molecular function of the SPX domain will be a crucial step towards a better understanding of phosphate sensing in eukaryotic cells. It may even provide a useful tool in overcoming Pi limitation in plant agriculture.
Status
Administrative delay for the defence
URL http://structplantbio.org
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