Electron Microscopy Practical Course

22 - 26 November 2021

Dr. Christel Genoud, Electron Microscopy Facility, University of Lausanne

Christel Genoud, Dr., Head of the Electron Microscopy Facility (EMF), University of Lausanne (UNIL)
Jean Daraspe, Scientific expert, EMF, UNIL
Olivia Muriel Lopez, Dr., Scientific expert (in-situ CLEM, tomography), EMF & UNIL
Irina Kolotuev, Senior scientist UNIL, expert (array tomography)
Céline Loussert, Senior scientist UNIFR, expert (cryo-immuno)
Graham Knott, Prof., Head of bio-EM facility EPFL, EPFL
Amanda Lewis, Dr., expert (CLEM for neurodegeneration), EPFL
Romain Guiet, Dr., imaging specialist, BIOP, EPFL
Alexandre Myasnikov, Dr., Head of Dubochet Center for Imaging, EPFL
Antonio Mucciolo, EMF, UNIL
Damien De Bellis, EMF, UNIL

The course with take place at the Electron Microscopy Facility of the University of Lausanne.

9h15-12h00: Lectures & Student's talks - ROOM 2451

13h15-16h45: Hands-on demonstrations & Work on your own sample - ROOM 1219

Goals:
- To identify the best EM method for each student samples/questions
- To discover which EM techniques that are available at the EMF could forward your research
- To identify how molecules can be localized in cells or tissues. - To learn the basics of EM image acquisition, analysis and reconstruction
- To understand basic SEM and TEM operating principles
- To gain exposure to various methods of preparing biological samples for EM together with the advantages and disadvantages related to each of these methods

The course with take place at the Electron Microscopy Facility of The University of Lausanne.

9h15-12h00: Lectures & Student's talks - ROOM 2451

13h15-16h45: Hands-on demonstrations & Work on your own sample - ROOM 1219

Goals:
- To identify the best EM method for each student samples/questions
- To discover which EM techniques that are available at the EMF could forward your research
- To identify how molecules can be localized in cells or tissues. - To learn the basics of EM image acquisition, analysis and reconstruction
- To understand basic SEM and TEM operating principles
- To gain exposure to various methods of preparing biological samples for EM together with the advantages and disadvantages related to each of these methods

Topics covered:
- TEM, transmission electron microscopy
- TEM sample preparation: chemical and high-pressure freezing procedures
- SEM, scanning electron microscopy
- SEM sample preparation
- Volume EM: TEM- tomography, Focused Ion Beam-SEM, Array Tomography
- Ultramicrotomy, resin sections
- Cryo-ultramicrotomy, the Tokuyasu technique
- Image processing, volume reconstruction, and modelling

- TEM, transmission electron microscopy
- TEM sample preparation: chemical and high-pressure freezing procedures
- SEM, scanning electron microscopy
- SEM sample preparation
- Volume EM: TEM- tomography, Focused Ion Beam-SEM, Array Tomography
- Ultramicrotomy, resin sections
- Cryo-ultramicrotomy, the Tokuyasu technique
- Image processing, volume reconstruction, and modelling

University of Lausanne, Electron Microscopy Facility, Room TBA

The course is organized in collaboration with the CUSO doctoral programs in Molecular Plant Sciences (CUSO MPS), Microbiology (CUSO Microbiology) and StarOmics (CUSO StarOmics)

IMPORTANT INFORMATION concerning COVID safety measures: participants must provide a certificate of vaccination or a negative test to participate to the course.

Reimbursements:
Reimbursements for CUSO students: Train ticket, 2°class, half-fare from your institution to the place of the activity.
NO reimbursement for your meal expenses.

CUSO StarOmics students:
Reimbursement form attached with original tickets has to be sent to the StarOmics coordinator Corinne Dentan at University of Lausanne, CIG, Le Génopode, 1015 Lausanne.

NEW from 2021: Reimbursement of your travel tickets can be asked online through your MyCUSO.
See HERE for the procedure. For any question concerning reimbursement please contact the CUSO StarOmics coordinator Corinne Dentan.

Other CUSO students:
Please contact the coordinator of your program.

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