Detailed information about the course

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Title

CRISPR Cas9

Dates

12-14 March 2019

Lang EN Workshop language is English
Responsable de l'activité

Pilar Junier

Organizer(s)
Speakers

Prof. Anil Wipat, Newcastle University;
Dr. Valeria Verrone, Newcastle University

Description

An introduction to CRISPR-Cas9: A focus on bacteria

 

Although a relatively recent discovery, CRISPR technology is beginning to revolutionise biotechnology. This workshop is designed for beginners to the field to introduce scientists to the world of CRISPR by providing a summary of the fundamentals. The workshop will comprise lectures, workshops and laboratory-based exercises. The lectures cover the history and theory of CRISPR, how the technology works, the wide variety of CRISPR applications, and approaches to carrying out your own CRISPR experiment, with a special focus on engineering bacteria. A computational workshop will demonstrate how guide RNA for CRISPR experiments can be designed. The practical component of the course in the lab will demonstrate how dCas9 based CRISPR can be used to knock down bacterial gene expression.

Program

Trainers: Anil Wipat & Valeria Verrone

 

Program

 

Tuesday 12th March 2019 – Room E301

09:00-10:30
Lectures on background of the technology, underlying biology, practical issues and applications focusing
on applications of CRISPR-Cas9 in microbiology. 

10:30-11:00 Coffee break

11:00-12:00
Interactive session about the practical: creating a KO cadA using CRISPR on Escherichia coli

12:00-14:00
Lunch break (lunch for the group at the cafeteria of UniMail)

14:00-15:00
Computational practical in Guide RNA (please bring your computer)

15:00-15:30
Coffee break

15:30-17:00
Cont. Computational practical in Guide RNA

18:00
Dinner with the speakers (please let us know in advance if you would like to join for this activity).

 

Wednesday 13th March 2019 – Room TP4 D008 

09:00-10:30
Preparing competent cells 

10:30-11:00
Coffee break

11:00-12:00 Cont. Preparing competent cells.

12:00-14:00
Lunch break (lunch for the group at the cafeteria of UniMail). 

14:00-15:00
Transforming the strains. 

15:00-15:30 C
offee break

15:30-17:00
Cont. Transforming the strains and discussion of the theoretical steps that follow.

 

Thursday 14th March 2019 – Room TP4 D008 

09:00-10:30
Assays to verified the phenotype.

10:30-11:00
Coffee break

11:00-12:00
Cont. Assays to verified the phenotype and discussion of the overall activity.

Location

Neuchâtel

Information
Expenses

Reimbursements for CUSO StarOmics students

Train ticket, 2°class, half-fare from your institution to the place of the activity, hotel if necessary (1 night + breakfast: up to 90 CHF per day per person).
NO reimbursement for your meal expenses

Reimbursement form attached with original tickets has to be sent to the StarOmics coordinator Corinne Dentan at University of Lausanne, Centre Intégratif de Génomique, Le Génopode, 1015 Lausanne.

For any question concerning reimbursement please contact the CUSO StarOmics coordinator Corinne Dentan.

Places

15

Deadline for registration
Joint activity joint
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