Electron Microscopy Practical Course

1-5 March 2021

Christel Genoud, Dr., Electron Microscopy Facility, University of Lausanne

Christel Genoud, Dr., Electron Microscopy Facility, University of Lausanne
Jean Daraspe, Electron Microscopy Facility, University of Lausanne
Antonio Mucciolo, Electron Microscopy Facility, University of Lausanne
Damien De Bellis, Electron Microscopy Facility, University of Lausanne
- 2 external (to confirm)

The course with take place at the Electron Microscopy Facility of The University of Lausanne.

Mornings: lectures

Afternoon: hands-on demonstrations + work on your own sample Goals:
- To identify the best EM method for each student samples/questions
- To discover which EM techniques that are available at the EMF could forward your research
- To identify how molecules can be localized in cells or tissues. - To learn the basics of EM image acquisition, analysis and reconstruction
- To understand basic SEM and TEM operating principles
- To gain exposure to various methods of preparing biological samples for EM together with the advantages and disadvantages related to each of these methods

Topics covered:
- TEM, transmission electron microscopy
- TEM sample preparation: chemical and high-pressure freezing procedures
- SEM, scanning electron microscopy
- SEM sample preparation
- Volume EM: TEM- tomography, Focused Ion Beam-SEM, Array Tomography
- Ultramicrotomy, resin sections
- Cryo-ultramicrotomy, the Tokuyasu technique
- Image processing, volume reconstruction, and modelling

MORE INFORMATION WILL FOLLOW

University of Lausanne, Electron Microscopy Facility, Room TBA

The course is organized in collaboration with the CUSO doctoral programs in Microbiology (CUSO Microbiology) and StarOmics (CUSO StarOmics).

Reimbursements:

Reimbursements for CUSO Students: Train ticket, 2°class, half-fare from your institution to the place of the activity.
NO reimbursement for your meal expenses.

CUSO MPS Students:
Reimbursement form attached with original tickets has to be sent to the MPS coordinator Zoia Debora at University of Lausanne, Department of Molecular Plant Biology, Biophore - quartier UNIL-Sorge, 1015 Lausanne.
For any question concerning reimbursement please contact the CUSO MPS coordinator Debora Zoia.

Other CUSO Students:
Please contact the coordinator of your Program.

Deadline for Registration: 10 February 2021

12 places available for CUSO students (4 for each participating program)

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