Electron Microscopy Practical Course

10-14 October 2022

Christel Genoud, Dr., Electron Microscopy Facility, University of Lausanne

Christel Genoud, Dr., Head of the Electron Microscopy Facility (EMF), University of Lausanne (UNIL)
Jean Daraspe, Scientific expert, EMF, UNIL
Irina Kolotuev, Senior scientist UNIL, expert
Antonio Mucciolo, EMF, UNIL
Damien De Bellis, EMF, UNIL
Olivia Muriel Lopez, EMF, UNIL
Céline Loussert, senior scientist, UNIFR
Graham Knott, head of bio-EM facility, EPFL
Amanda Lewis, post-doc EPFL
Benjamin Boury Jamot, imaging specialist, CEC, CHUV
Alexandre Myasnikov, head of Dubochet Center for Imaging
Cristina Martin Olmos, head cryo-SEM, EPFL

The course with take place at the Electron Microscopy Facility of The University of Lausanne.

Mornings: lectures

Afternoon: hands-on demonstrations + work on your own sample

Goals:
- To identify the best EM method for each student samples/questions
- To discover which EM techniques that are available at the EMF could forward your research
- To identify how molecules can be localized in cells or tissues.
- To learn the basics of EM image acquisition, analysis and reconstruction
- To understand basic SEM and TEM operating principles
- To gain exposure to various methods of preparing biological samples for EM together with the advantages and disadvantages related to each of these methods

Topics covered:
- TEM, transmission electron microscopy
- TEM sample preparation: chemical and high-pressure f freezing procedures
- SEM, scanning electron microscopy
- SEM sample preparation
- Volume EM: TEM- tomography, Focused Ion Beam-SEM, Array Tomography
- Ultramicrotomy, resin sections - Cryo-ultramicrotomy, the Tokuyasu technique
- Image processing, volume reconstruction, and modelling

Tasks for students:

> On Monday morning, each student has 3 minutes (1-2 slides) to explain his needs in electron microscopy in the frame of the PhD work. If students wish to bring their own samples for tests, please contact [email protected]

> PREMILINARY PROGRAM (PDF)

University of Lausanne, Electron Microscopy Facility

> FLYER (PDF)

The course is organized in collaboration with the CUSO doctoral programs in Microbiology (CUSO Microbiology) and StarOmics (CUSO StarOmics).

Lectures (mornings): Room 340.1, AMPHIPÔLE, UNIL.

Reimbursements:

Reimbursements for CUSO MPS Students:
- Train ticket, 2°class, half-fare from your institution to the place of the activity.

NEW from 2021: Reimbursement of your travel tickets can be asked online through your MyCUSO. See HERE for the procedure.
For any question concerning reimbursement please contact the CUSO MPS coordinator Debora Zoia

Other CUSO Students:
Please contact the coordinator of your Program.

Deadline for registration: 26 September 2022
12 places available for CUSO students (4 for each participating program)

** 50 CHF of cancelation fees will be demanded for cancelations after the registration deadline **

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