Electron Microscopy (EM) and Cryo-EM - Practical Course

3-4 & 10-11 November 2025

Dre Christel Genoud, Electron Microscopy Facility, UNIL

Dre Christel Genoud, UNIL
Jean Daraspe, UNIL
Antonio Mucciolo, UNIL
Damien De Bellis, UNIL
2-4 intervenant·es externes

Mornings: lectures
Afternoon: hands-on demonstrations + work on your own sample
2 days focusing on classic EM, 2 days on Cryo EM

Goals:
- To identify the best EM method for each student samples/questions
- To discover which EM techniques that are available at the EMF could forward your research
- To identify how molecules can be localized in cells or tissues.
- To learn the basics of EM image acquisition, analysis and reconstruction
- To understand basic SEM and TEM operating principles
- To gain exposure to various methods of preparing biological samples for EM together with the advantages and
disadvantages related to each of these methods

Topics covered:
- TEM, transmission electron microscopy
- cryoTEM
- TEM sample preparation: chemical and high-pressure freezing procedures
- SEM, scanning electron microscopy
- SEM sample preparation
- Volume EM: TEM- tomography, Focused Ion Beam-SEM, Array Tomography
- Ultramicrotomy, resin sections
- Cryo-ultramicrotomy, the Tokuyasu technique
- Image processing, volume reconstruction, and modelling

On the first day of activity, each student has 3 minutes (1-2 slides) to explain his needs in electron microscopy in the frame of the PhD work. 

More Information will follow

UNIL

Reimbursements for CUSO MPS Students: Train ticket, 2°class, half-fare from your institution to the place of the activity.

Reimbursement of your travel tickets can be asked online through your MyCUSO.

REGISTRATION OPEN

 Deadline for registration: 20 October 2025

12 places available for CUSO students (4 for each participating program) Minimum number of participants: 10

 For cancellations after the deadline of registration or no show:  150 CHF administrative fees 

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