Detailed information about the course
Title | Invited seminar: How do Hantaviruses enter and exit Human cells |
Dates | August 29, 2014 |
Responsable de l'activité | Stefan Kunz |
Organizer(s) | Prof. Stefan Kunz, UNIL |
Speakers | Prof. Nicole Tischler, Head, Molecular Virology Laboratory |
Description | INVITED SEMINARAugust 29, 2014, 09:00CHUV/ Institute of Microbiology (IMUL) Room 502
SUMMARYHantaviruses cause worldwide human disease and are classified by the CDC as category A pathogens. As other members of the Bunyaviridae family, they are single stranded RNA viruses of negative polarity that contain a lipid envelope membrane. Yet, little information is available about their cell entry and exit mechanisms. In order to infect cells, hantavirus Gn and Gc envelope glycoproteins mediate cell attachment and virus-cell membrane fusion. In this seminar, work will be presented concerning different fusion intermediate steps, such as Gc activation, Gc multimerization, interaction with liposomes as well as late steps of the fusion mechanism cascade. For cell exit, hantaviruses must acquire a lipid membrane from the host cell. Here, data will be shown that shows hantavirus Gn and Gc self-assembly into virus-like particles (VLPs), suggesting that Gn and Gc glycoproteins drive virus budding without the need of other viral components.
ROUND-TABLE DISCUSSION for Ph.D studentsInsights into the virus-cell membrane fusion mechanism of bunyaviruses and class II fusion proteins During the round table discussion, papers will be analyzed that use classical biochemical and cell biological techniques to approach the characterization of different fusion proteins. One paper focuses on late intermediate stages of the fusion mechanism mediated by an alphavirus while the other on the fusion activation of a phlebovirus fusion protein. These papers will prepare the students to follow Dr. Tischler’s seminar on hantavirus glycoproteins. The goal is to gain current insights into viral fusion with the envelope proteins of hantavirus in comparison with other bunyaviruses, including phleboviruses.
Format: The participating students will read the speaker’s papers and discuss novel concepts of viral host cell entry. Location: CHUV, IMUL, Rue du Bugnon 48, room 502, Schedule: 14:00 – 16: 00 Organizer: Prof. Stefan Kunz Publications to read: Acid-activated structural reorganization of the Rift Valley Fever Virus Gc Fusion protein Review: A novel membrane fusion protein family in Flaviviridae The interaction of Alphavirus E1 protein with Exogenous Domain III Review: Hantavirus Gn and Gc envelope Glycoproteins
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Location |
CHUV/IMUL |
Map | |
Credits | 0.25 |
Information | |
Places | 6 |
Deadline for registration | 28.08.2014 |